Full Publication List on Google Scholar
Full Publication List on Pubmed
2024
D Schuftan, YKG Kooh, J Guo, Y Sun, L Aryan, B Stottlemire, C Berkland, GM Genin, N Huebsch. Dynamic control of contractile resistance to iPSC-derived micro-heart muscle arrays. J Biomed Mater Res A. 2024; 112(4): 534-48. doi: 10.1002/jbm.a.37642.
DW Simmons, GM Malayath, DR Schuftan, J Guo, K Oguntuyo, G Ramahdita, Y Sun, SD Jordan, MK Munsell, B Kandalaft, M Pear, SL Rentschler, N Huebsch. Tissue geometry and plakophilin 2 regulate electrophysiology of iPSC-derived micro-heart muscle. APL Bioeng. 2024, 8(1): 016118.doi: 10.1063/5.0160677. Featured Article.
2023
J Guo, H Jiang, D Schuftan, J Moreno, D Bhagavan, L Aryan, G Ramahdita, JR Silva, N Huebsch. Mechanical resistance to micro-heart tissue contractility unveils early structural and functional pathology in iPSC-models of hypertrophic cardiomyopathy. In Revision. Preprint available: doi.org/10.1101/2023.10.30.564856.
LG Woodhams*, J Guo*, JJ Boyle, D Schuftan, KM Pryse, EL Elson**, N Huebsch**, GM Genin**.* Equal first-authors. **Co-corresponding authors. Virtual blebbistatin: a robust software approach to reconstructing calcium-contraction coupling in iPSC-cardiomyocytes. Proc Natl. Acad. Sci. USA. 2023; 120(38): e2212949120. doi: 10.1073/pnas.2212949120
DW Simmons, DR Schuftan, G Ramahdita, N Huebsch. Hydrogel-Assisted Double Molding Enables Rapid Replication of Stereolithographic 3D Prints for Engineered Tissue Design. ACS Appl. Mater. Interfaces. 2023. doi.org/10.1021/acsami.3c02279
C Wang*, G Ramahdita*, G Genin, N Huebsch**, Z Ma**. Dynamic mechanobiology of cardiac cells and tissues: Current status and future perspective. Biophysics Rev. 2023. *Equal first-authors. **Co-corresponding authors. doi.org/10.1063/5.0141269
2022
N Huebsch. Collective organization from cellular disorder. Biophys J. 2022. doi.org/10.1016/j.bpj.2022.10.010
K Oguntuyo*, D Schuftan*, J Guo, DW Simmons, D Bhagavan, JD Moreno, EW Miller, JR Silva**, N Huebsch**. Robust, Automated Analysis of Action Potential and Calcium Transient Morphology in iPSC-Derived Micro-Heart Muscle for In Vitro Assessment of Drug-Induced Toxicity. Tissue Eng Part C Methods. 2022. *Equal first-authors. **Co-corresponding authors. doi.org/10.1089/ten.TEC.2022.0053
DW Simmons, N Huebsch. “iPSC-Derived Micro-Heart Muscle for Medium Throughput Pharmacology and Pharmacogenomic Studies.” Cardiac Tissue Engineering: Methods and Protocols, 2nd. Ed. L.D. Black III and K.L.L. Coulomb Ed. 2022. doi.org/10.1007/978-1-0716-2261-2_8
N Huebsch*, B Charrez*, B Siemons, SC Boggess, S Wall, V Charwat, KH Jæger, D Cleres, A Telle, FT Lee-Montiel, N Jeffreys, N Deveshwar, A Edwards, J Serrano, M Snuderl, A Stahl, A Tveito, EW Miller, KE Healy. Metabollically-Driven Maturation of Human Induced Pluripotent Stem Cell Derived Heart-On-A-Chip. Nat Biomed Eng: 2022; 6, 372–388. doi.org/10.1038/s41551-022-00884-4. *Equal first authors.
JD Moreno, D Bhagavan, A Li, N Huebsch, S Cresci, JR Silva. Pulsus alternans in cardiogenic shock recapitulated in single cell fluorescence imaging of a patient’s cardiomyocyte. Circ. Heart Fail 2022; 15:e008855. doi.org/ 10.1161/CIRCHEARTFAILURE.121.008855
2021
X Tan, E Jain, M Barcellona, E Morris, S Neal, M Gupta, J Buchowski, M Kelly, L Setton, N Huebsch. Integrin and syndecan binding peptide-conjugated alginate hydrogel for modulation of nucleus pulposus cell phenotype. Biomaterials. 2021. doi.org/10.1016/j.biomaterials.2021.121113
Z Clapacs, S Neal, D Schuftan, X Tan, H Jiang, J Guo, J Rudra, N Huebsch. Biocompatible and Enzymatically Degradable Gels for 3D Cellular Encapsulation under Extreme Compressive Strain. Gels. 2021;7(3):101. doi:10.3390/gels7030101
J Guo, H Jiang, K Oguntuyo, B Rios, Z Boodram, N Huebsch. Interplay of Genotype and Substrate Stiffness in driving the Hypertrophic Cardiomyopathy Phenotype in iPSC-Micro-Heart Muscle Arrays. Cellular and Molecular Bioengineering. 2021. Young Innovators Special Issue. doi: doi.org/10.1007/s12195-012-00684-x
FT Lee-Montiel, A Laemmle, L Dumont, CS Lee, N Huebsch, V Charwat, H Okochi, MJ Hancock, B Siemons, S Boggess, I Goswami, E Miller, H Willenbring, KE Healy. Integrated hiPSC-based liver and heart microphysiological systems predict unsafe drug-drug interactions. Frontiers in Pharmacology. 2021; 12: 977. doi: 10.3389/fphar.2021.667010
E Jain, S Neal, H Graf, X Tan, R Balasubramaniam, N Huebsch. Copper-Free Azide–Alkyne Cycloaddition for Peptide Modification of Alginate Hydrogels. ACS Applied Bio Materials. 2021. doi: 10.1021/acsabm.0c00976
J Guo, DW Simmons, G Ramahdita, MK Munsell, K Oguntuyo, B Kandalaft, B Rios, M Pear, D Schuftan, H Jiang, SP Lake, GM Genin, N Huebsch. Substrate Grafted iPSC-Derived Micro Heart Muscles to Investigate Effects of Mechanical Loading on Cardiac Physiology. ACS Biomaterials Science & Engineering. 2021; 7(7): 2973-89. doi: 10.1021/acsbiomaterials.0c00318
2020
J Guo, N Huebsch. Modeling the Response of Heart Muscle to Mechanical Stimulation in vitro. Current Tissue Microenvironment Reports. 2020. doi: 10.1007/s43152-020-00007-8
2019
N Huebsch. Translational Mechanobiology: Designing Synthetic Hydrogel Matrices for Improved In Vitro Models and Cell-Based Therapies. Acta Biomaterialia. 2019; 94: 97-111. doi: 10.1016/j.actbio.2019.05.055
P Loskill, N Huebsch. Engineering Tissues from Induced Pluripotent Stem Cells. Tissue Eng. A. 2019. 25(9-10): 707-10. doi: 10.1089/ten.tea.2019.0118
2018
SC Boggess, SS Gandhi, BA Siemons, N Huebsch, KE Healy, EW Miller. New Molecular Scaffolds for Fluorescent Voltage Indicators. ACS Chem Biol. 2019; 14(3): 390-6. doi: 10.1021/acschembio.8b00978
A Tveito, KH Jæger, N Huebsch, B Charrez, AG Edwards, S Wall, KE Healy. Inversion and computational maturation of drug response using human stem cell derived cardiomyocytes in microphysiological systems. Sci Rep 2018; 8(1): 17626. doi: 10.1038/s41598-018-35858-7
Z Ma*, N Huebsch*, S Koo*, MA Mandegar, B Siemons, BR Conklin, CP Grigoropolous, KE Healy. Synergy of MYBPC3 Deficiency and Mechanical Overload Leads to Contractile Deficits in Engineered Cardiac Tissue. Nat Biomed. Eng. 2018; 2(12): 955-67. doi: 10.1038/s41551-018-0280-4 * Equal first authors.
P Hoang, N Huebsch, SH Bang, Siemons BA, Conklin BR, Healy KE, Ma Z, Jacquir S. Quantitatively Characterizing Drug-Induced Arrhythmic Contractile Motions of Human Stem Cell-Derived Cardiomyocytes. Biotechnol. Bioeng. 2018; 115(8): 1958-70. doi: 10.1002/bit.26709
2017
LM Judge, JA Perez-Bermejo, A Truoung, AJ Ribeiro, JC Yoo, CL Jensen, MA Mandegar, N Huebsch, RM Kaake, PL So, D Srivastava, BL Pruitt, NJ Krogan, BR Conklin. A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. JCI Insight. 2017; 2(14). Pii: 94623. doi: 10.1172/jci.insight.94623
A Cipitria, K Both, S Schoenhals, DS Garske, K Schmidt-Bleek, A Ellinghaus, A Dienelt, A Peters, M Mehta, CM Madl, N Huebsch, DJ Mooney, GN Duda. In-situ tissue regeneration through SDF-1a driven cell recruitment and stiffness-mediated cell differentiation in a rat femoral critical-sized defect. Acta Biomater. 2017; pii: S1742-7061(17)30470-1. doi: 10.1016/j.actbio.2017.07.032
2016
N Huebsch, P Loskill, N Deveshwar, CI Spencer, LM Judge, MA Mandegar, C Fox, TMA Mohammed, Z Ma, A Mathur, AM Sheehan, M Saxton, J Yoo, D Srivastava, TA Desai, P So, KE Healy, BR Conklin Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses. Sci. Rep. 2016; 6: 24726. doi: 10.1038/srep24726
MA Mandegar, N Huebsch, EB Frolov, E Shin, LM Judge, Y Miyaoka, A Truong, AH Chan, CI Spencer, P So, Qi LS, Conklin BR. CRISPR Interference Efficiently Induces Gene Knockdown and Models Disease in iPSCs. Cell Stem Cell. 2016; 18(4): 541-53. doi: 10.1016/j.stem.2016.01.022
O Chaudhuri, L Guo, D Klumpers, M Darnell, S Bencherif, J Weaver, N Huebsch, HP Lee, E Lippens, GN Duda, DJ Mooney. Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nat. Mater 2016; 15(3): 326-34. doi: 10.1038/nmat4489
2015
N Huebsch, E Lippens, K Lee, ST Koshy, MC Darnell, R Desai, M Mehta, CM Madl, M Xu, O Chaudhuri, X Zhao, KA Alim, A Mammato, DE Ingber, GN Duda, DJ Mooney. Matrix elasticity of void-forming hydrogels controls transplanted-stem-cell-mediated bone formation. Nat Mater 2015; 14(12): 1269-77. doi: 10.1038/nmat4407
Z Ma, J Wang, P Loksill, N Huebsch, S Koo, FL Svedlund, NC Marks, MA Finnegan, CP Grigoropoulos, BR Conklin, KE Healy. Self-organizing cardiac microchamber mediated by geometric confinement. Nat Commun. 2015; 6: 7413. doi: 10.1038/ncomms8413
A Mathur, P Loskill, K Shao, N Huebsch, S Hong, SG Marcus, N Marks, M Mandegar, BR Conklin, LP Lee, KE Healy. Human iPSC-based Cardiac Microphysiological System For Drug Screening Applications. Sci. Rep. 2015; 5: 8883. doi: 10.1038/srep08883
N Huebsch*, P Loskill*, MA Mandegar, NC Marks, AS Sheehan, Z Ma, AA Mathur, TN. Nguyen, JC Yoo, LM Judge, CI Spencer, AC Chukka, CR Russell, PL So, BR Conklin, KE Healy. Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales. Tissue Eng Part C Methods 2015; 21(5): 467-79. doi: 10.1089/ten.TEC.2014.0283 *Equal first authors.
2009-2014
N Huebsch*, C Kearney*, X Zhao*, J Kim, C Cezar, Z Suo, DJ Mooney. Ultrasound-triggered disruption and self-healing of reversibly cross-linked hydrogels for drug delivery and enhanced chemotherapy. Proc. Natl. Acad Sci. USA, 2014; 111(27): 9762-7. doi: 10.1073/pnas.1405469111 * Equal first authors.
JD Boerckel, BA Uhrig, NJ Willett, N Huebsch, RE Guldberg. Mechanical Regulaton of Vascular Growth and Tissue Regeneration In Vivo. Proc. Natl. Acad. Sci. USA. 2011; 108(37): E674-80. doi: 10.1073/pnas.1107019108
YM Kolambkar, JD Boerckel, KM Dupont, M Bajin, N Huebsch, DJ Mooney, DW Hutmacher, RE Guldberg. Spatiotemporal delivery of bone morphogenic protein enhances functional repair of segmental bone defects. Bone 2011; 49(3): 485-92. doi: 10.1016/j.bone.2011.05.010
X Zhao, J Kim, C Cezar, N Huebsch, K Bouhadir, DJ Mooney. Active Scaffolds for On-Demand Drug and Cell Delivery. Proc Natl Acad Sci USA 2011; 108(1): 67-72. doi: 10.1073/pnas.1007862108
N Huebsch, PR Arany, AS Mao, D Shvartsman, OA Ali, SA Bencherif, J Rivera-Feliciano, DJ Mooney. Harnessing Traction-Mediated Manipulation of the Cell-Matrix Interface to Control Stem Cell Fate. Nat Mater. 9(6): 518-26. doi: 10.1038/nmat2732
N Huebsch, DJ Mooney. Inspiration and application in the evolution of biomaterials. Nature 2009; 462 (Insight Supplement): 1-7. doi: 10.1038/nature08601
OA Ali, N Huebsch, L Cao, G Dranoff, DJ Mooney. Infection-mimicking materials to program dendritic cells in situ. Nat Mater 2009; 8(2): 151-8. doi: 10.1038/nmat2357